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Defrosting the Freezer

Climate change and environmental science research and resources from Dr Robert Sparkes, Manchester Metropolitan University

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Recent Posts

  • Sharing our Standard Operating Procedures
  • Rapid carbon accumulation at a saltmarshrestored by managed realignment exceeded carbon emitted in direct site construction
  • Characterization of diverse bacteriohopanepolyols in a permanently stratified, hyper-euxinic lake
  • The quantification of di-octyl terephthalate and calcium carbonate in polyvinyl chloride using Fourier transform-infrared and Raman spectroscopy
  • Methanotroph-derived bacteriohopanepolyol signatures in sediments covering Miocene brown coal deposits

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Category: SOP

015 Total Suspended Solids

BC_015 Total Suspended Solids v1.0Download

Filtration of water samples to remove and measure suspended solids.

Posted on 27 September, 202427 September, 2024Categories SOPLeave a comment on 015 Total Suspended Solids

014 Nutrient Extraction

BC_014 Nutrient extraction v1.0Download

Extracting nutrients from sediment using de-ionised water.

Posted on 27 September, 202427 September, 2024Categories SOPLeave a comment on 014 Nutrient Extraction

013 Dilute Acid Digestion

BC_013 Dilute acid digest v1.0Download

Extracting elements from sediments using dilute (0.43 M) nitric acid.

Posted on 27 September, 202427 September, 2024Categories SOPLeave a comment on 013 Dilute Acid Digestion

012 BHP Extraction and Separation

BC_012 BHP Extraction and SeparationDownload

Extracting lipids from dried sediments. Separating bacteriohopanepolyol (BHP) molecules from the total lipid extract. Acetylating BHP molecules and preparation for LC-MS analysis.

Citation: O’Beirne et al., 2022.

Posted on 27 September, 202427 September, 2024Categories SOPLeave a comment on 012 BHP Extraction and Separation

011 Dry Bulk Density

BC_011 Dry Bulk Density v1.0Download

Sampling and measurement of the dry bulk density of surface sediments.

Posted on 27 September, 202427 September, 2024Categories SOPLeave a comment on 011 Dry Bulk Density

010 Sediment Core Sampling

BC_010 Sediment Core sampling v1.0Download

Collection of 1 metre sediment cores using a steel auger.

Posted on 27 September, 202427 September, 2024Categories SOPLeave a comment on 010 Sediment Core Sampling

009 Sediment Surface Sampling

BC_009 Surface sampling v1.0Download

Collection of the top 15 cm of sediment using a steel auger.

Posted on 27 September, 202427 September, 2024Categories SOPLeave a comment on 009 Sediment Surface Sampling

008 Preparing Elemental Analysis Foils

BC_008 Preparing Elemental Analysis foils v1.0Download

Adding sediment to tin foil cups prior to quantification of C, N, O, H or S via Elemental Analysis.

Posted on 27 September, 202427 September, 2024Categories SOPLeave a comment on 008 Preparing Elemental Analysis Foils

007 Furnacing Organic Matter

BC_007 Furnacing organic matter v1.2Download

Removal of organic matter from sediments using a moderate-temperature furnace.

Posted on 27 September, 202427 September, 2024Categories SOPLeave a comment on 007 Furnacing Organic Matter

006 Acid Decarbonation

BC_006 Acid decarbonation v1.0Download

Removal of carbonate minerals (inorganic carbon) using hydrochloric acid.

Note: this process also removes some organic matter (publication in review).

Posted on 27 September, 202427 September, 2024Categories SOPLeave a comment on 006 Acid Decarbonation

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